Curcin和curcin C与腺嘌呤互作位点的比较分析
DOI:
作者:
作者单位:

作者简介:

通讯作者:

基金项目:

国家自然科学基金(31870315)


Comparative analysis of the interaction sites of curcin, curcin C with adenine
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
    摘要:

    麻疯树核糖体失活蛋白curcin和curcin C均具有抗肿瘤活性,但后者的活性水平显著高于前者,为了探索造成这一差异的结构基础,本文采用在线的同源建模预测软件SWISSMODEL,对两种麻疯树核糖体失活蛋白curcin、curcin C的三维结构模型进行预测,采用SYBYL对预测模型进行能量最小化优化,采用PROCHECK、VERIFY 3D和ERRAT软件对优化前后的模型进行质量评估,随后采用AutoDock软件将预测的模型与腺嘌呤进行分子对接分析.结果显示,两种蛋白采用与Ricin A类似的方式与腺嘌呤相互作用,但在相互作用的氨基酸残基种类、数量以及形成的氢键和疏水相互作用上还是存在差异,其中,curcin C与腺嘌呤具有最强的结合能力,curcin则最低.

    Abstract:

    The ribosome-inactivating proteins of Jatropha curcas curcin and curcin C have antitumor activity, but the activity level of the latter is significantly higher than the former. In order to explore the structural basis for this difference, the online homology modeling prediction software SWISSMODEL was used to predict the threedimensional structural models of the ribosomal inactivating proteins curcin and curcin C. SYBYL was used to optimize the energy of the prediction model, and PROCHECK, VERIFY 3D and ERRAT software were used to evaluate the quality of the model before and after the optimization, and then AutoDock software was used to analyze the predicted model and adenine for molecular docking. The results show that the two proteins interacted with adenine in a similar way to Ricin A, but there were differences in the types and number of amino acid residues that interact, as well as the hydrogen bonds and hydrophobic interactions formed. Among them, curcin C had the strongest binding ability with adenine, and curcin had the lowest.

    参考文献
    相似文献
    引证文献
引用本文

引用本文格式: 吴朋,邓聿杉,陈放,徐莺. Curcin和curcin C与腺嘌呤互作位点的比较分析 [J]. 四川大学学报: 自然科学版, 2020, 57: 1186~1192.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
历史
  • 收稿日期:2020-03-09
  • 最后修改日期:2020-04-24
  • 录用日期:2020-05-14
  • 在线发布日期: 2020-12-02