过表达甘油激酶三角褐指藻藻株富集油脂的研究
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国家自然科学基金(40976092);四川省电科院项目(13H1138)


Study on lipid accumulation of Phaeodactylum tricornutum strain overexpressing glycerol kinase
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    摘要:

    为了得到高产并符合生物柴油标准的微藻油脂的藻株,本文研究了在外源甘油存在的情况下野生型(WT)三角褐指藻(Phaeodactylum tricornutum),甘油激酶过表达藻株(GKOE)以及甘油激酶RNA干扰藻株(GKRNi)在添加浓度为20 mmol/L外源甘油的培养基中的生长情况、油脂产量以及脂肪酸组成.结果显示:(1)甘油兼养的GKOE藻细胞浓度可达到2.23×107个/mL,明显高于甘油兼养的WT的1.79×107个/mL和GKRNi的1.78×107 个/mL;(2)甘油兼养的GKOE油脂含量可达到31.73%,比甘油兼养的WT和GKRNi分别高了27.20%和26.63%;(3)尼罗红染色显示甘油兼养的GKOE荧光强度比甘油兼养的WT和GKRNi分别高16.26%和17.83%;(4)甘油兼养的GKOE总脂肪酸产量可达到0.052 mg/mL,比甘油兼养的WT和GKRNi高了36.84%和10.64%,说明其油脂适合于生物柴油开发.

    Abstract:

    In order to obtain high-yield algae strains that meet biodiesel standards, the growth, lipid yield and fatty acid composition of Phaeodactylum tricornutum wild-type(WT), Phaeodactyllum tricornutum with glycerol kinase overexpression (GKOE) and glycerol kinase RNA interfered(GKRNi), which were cultured in a medium supplemented with 20 mmol/L exogenous glycerol, were investigated. The results showed that: (1) the cell concentration of GKOE in the medium with exogenous glycerol could reach 2.23×107 /mL, significantly higher than WT(1.79×107 /mL) and GKRNi(1.78×107 /mL) in the medium with exogenous glycerol; (2) the lipid content of GKOE in the medium with exogenous glycerol was up to 31.73%, which was much higher than WT(27.20%) and GKRNi(26.63%) in the medium with exogenous glycerol; (3) the fluorescence intensity of GKOE in the medium with exogenous glycerol was 16.26% and 17.83% higher than WT and GKRNi in the medium with exogenous glycerol by Nile red staining, respectively; (4) the total fatty acid yield of GKOE reached 0.052 mg/mL, which was 16.26% and 17.83% higher than WT and GKRNi in the medium with exogenous glycerol, and suitable for biodiesel development.

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引用本文格式: 侯兴国,方琰,白欢,胡莎莎,卿人韦,兰利琼. 过表达甘油激酶三角褐指藻藻株富集油脂的研究 [J]. 四川大学学报: 自然科学版, 2020, 57: 1209~1216.

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  • 收稿日期:2020-01-09
  • 最后修改日期:2020-05-19
  • 录用日期:2020-05-27
  • 在线发布日期: 2020-12-02