磷脂酶D产生菌株的筛选及其转磷脂化反应特性
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Screening of Phospholipase D producing Strains and its transphosphatidylation activity
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    采用磷脂平板筛选法,从榨油坊附近的土样中筛选到1株能够降解磷脂的菌株SNUPLD-6,实验表明SNUPLD-6产的磷脂酶含有磷脂酶D(PLD).经细菌形态特及ITS序列分析,鉴定其为地霉属的白地霉(Geotrichum candidum).对该菌的生长条件及该菌产的PLD的反应条件进行研究,实验结果表明:该菌株的最适生长温度为30℃,最适PH为6,最适碳源为葡萄糖,最适氮源为牛肉膏和蛋白胨各50%.该菌产PLD最适发酵时间为3d,为胞外酶,该PLD的转磷脂化反应最适反应温度为32℃,最适pH为5.5,最适的反应缓冲液为pH为5.5的0.02M的醋酸-醋酸钠缓冲液,最适的金属离子激活剂为Ca2+,Zn2+.

    Abstract:

    Using soybean Phospholipids as substrates,one strain which expressed Phospholipase was isolated from soil near oil mill ,the Phospholipase produce by SNUPLD-6 was identified which contain Phospholipase D . According to its mor -phological characteristics and ITS sequence , The strain SNUPLD-6 was identified as Geotrichum candidum. study on the Conditions for the growth of the bacteria and the PLD reaction conditions which expressed by SNUPLD-6. The results showed that its optimum growthtemperature is 30℃ .its optimum growth pH value is 6.0,its optimum carbon source is glucose,its optimum nitrogen source for the beef extract and peptone 50% each. the optimal fermentation time of phospholipase D producing is 3d, its optimum transphosphatidylation-temperature is 32℃. its optimum transphosphatidylation pH value is 6.0,its optimum reaction buffer for pH=5.5 0.02M HAc -NaAC buffer, its Optimal metalion activator as Ca2 +, Zn2 +.

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引用本文格式: 石创,王一丁,马沁沁,袁向华. 磷脂酶D产生菌株的筛选及其转磷脂化反应特性 [J]. 四川大学学报: 自然科学版, 2016, 53: 215~220.

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  • 收稿日期:2014-11-21
  • 最后修改日期:2015-05-28
  • 录用日期:2015-06-21
  • 在线发布日期: 2016-05-30