This research aimed to establish a high confident list of mitochondrial genes with bioinformatics methods. Bayesian model of Mitochondrial gene function network are established by evidences of 14 integrated genome and mitochondrial level heterogeneous omics. The functions of Several mitochondrial genes are determined by new mitochondriarelated KEGG pathway components which are predicted in this network. 78 high confident mitochondriarelated KEGG pathway components have been doped out.